Vitamin D3 (Cholecalciferol)

Price of the test:
Please enquire our customer service at

Acceptable Matrices:
Our accreditation covers: milk, milk powders, infant formula

Limit of Detection:
Any concentration greater than 2 µg/100 g is detectable.

Sample Size Requirements:
50 grams of sample is needed.


Reportable Units:
µg/100 g

Turn Around Time:
10 Business Days

Is Urgent Service Available?
No (Enquire our customer service for special requirements at

Method Reference:
GB 5009.82-2016

Method Description:
Testing reports values for vitamin D3 (cholecalciferol). Following a saponification procedure to remove fat from the sample, the mixture is taken for liquid-liquid extraction into petroleum ether. The extract is then filtered and evaporated to dryness. The analyte is reconstituted in methanol and quantitated using reverse phase HPLC with a triple-quad mass spectrometer.

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